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Tissue Culture Research Center

  • Director: Kaoru Tohyama, M.D., Ph.D.

Outline and Characteristics of the Tissue Culture Research Center (TCRC)

The TCRC provides equipment, devices and nutrient media necessary for tissue culture-related research to staff and graduate students of the Kawasaki Medical School, Kawasaki University of Medical Welfare, and the Kawasaki College of Allied Health Professions. The TCRC is composed of a big common laboratory for tissue culture, five general laboratories equipped with working benches, which are offered to individual researchers on a one-year contract basis, six analysis rooms, a cleaning room, two cold rooms, a freezer room, two stockrooms, an office, and an isolated Biosafety Laboratory equipped with level II safety cabinets. Signed users can use clean benches, incubators, microscopes, centrifuges, flow-cytometers, etc. after making a reservation. The TCRC can supply culture flasks, pipettes, media, fetal calf sera and other reagents as needed and paid for by the user.

The avoidance of contamination by microorganisms is of fundamental importance in tissue culture. The TCRC offers a training course to instruct beginners on sterile techniques to prevent contamination. Trainees can apply for the course at any time. The TCRC is open 24 hours a day. However, the use of flow cytometers after office hours is only permitted when the methods of their use have been learned in a special training session.

The Biosafety Laboratory is where microorganisms with biosafety levels I-II are manipulated and stored. Individuals wishing to use this laboratory must complete an application form and submit it to the Biosafety Committee for approval. All experiments dealing with infectious materials must be performed within the Biosafety Laboratory. Users of this laboratory must obey the rules and keep the room and equipment clean.