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Tissue Biology & Electron Microscopy Research Center

  • Director:Kazunori Toida, M.D., Ph.D.

Outline of the Tissue Biology & Electron Microscopy Research Center

The Center is a centralized research and teaching facility which provides light, electron and scanning microscopy research of ultrastructures, serving a wide range of faculty and students of Kawasaki Medical School. The Center provides support and technical staff knowledgeable in instrument operation, as well as in techniques pertaining to specimen preparation, and offers training to potential users who wish to engage in research work using equipment located in the Center.

The facilities in the Center include a JEOL JEM-1400, a Hitachi H-7100 transmission electron microscope, a Hitachi S-3400N scanning electron microscope, and a JEOL JSM-6340F field emission scanning electron microscope. The S-3400N scanning electron microscope has HORIBA EX-250X-act energy dispersive spectroscopy systems for quantitative X-ray microanalysis.

The Center is equipped with various specimen preparation tools, including ion sputter coaters, a pure osmium coater, freeze-drying devices and critical point dryers for scanning electron microscopy, as well as Leica ultra-cut ultramicrotomes and vacuum evaporator for transmission electron microscopy. Other equipment in the Center includes two light microscopes (Nikon & KEYENCE) for fluorescence observation with several filter sets and TIRF (Total Internal Reflection Fluorescence) microscopy.

The center also contains a cryostat for processing frozen sections (Leica CM3050S), and automated slide processing systems for in situ hybridization and immunohistochemistry (Roche Ventana Discovery XT).

The ultra-rapid freezing machines, which freeze tissue samples within a few milliseconds, are available for electron microscopic studies on cytoplasm, cytoskeleton and membrane ultrastructures in high resolution. Frozen tissues for a replica technique of electron microscopy can be prepared using a Balzer's BAF-301 freeze-etching unit. For immunoelectron microscopy analyses, frozen sections are prepared in a cryo-ultramicrotome (Reichert-Jung SUPER NOVA).